Vitrification of preimplantation stages of mouse embryos
نویسندگان
چکیده
منابع مشابه
Which stage of mouse embryos is more appropriate for vitrification?
Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...
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Vitrification is a method of storing cells in a supercooled state, and is particularly useful for the cryostorage of embryos. With a supply of liquid nitrogen and simple apparatus, vitrification in the field situation is feasible and is potentially simpler and cheaper than the standard freezing processes. However, survival of embryos after vitrification has not yet proved better than after free...
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Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...
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Two-cell mouse embryos were cultured in vitro for different periods in a medium in which NaCl was partially replaced by LiCl at concentrations ranging from 1 to 30 mM. The relative cell number diminished according to increasing LiCl concentrations but the onset of blastulation was not affected, thus resulting in blastulae with fewer cells than normal and with a reduced or absent inner cell mass...
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Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28.6% o...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1993
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0980459